We have demonstrated that rotary jet spinning successfully produces microfiber networks of polymers accepted by the FDA for surgical implantation. Importantly, we can collect these fibers on spinning mandrels to rapidly generate tubular scaffolds that support cell culture. These scaffolds mimic the fibrillar architecture of human tissues. Prototypes for the rotary jet spinning system and bioreactor have demonstrated initial feasibility, but there is much room for optimization. One team will focus on optimizing the rotary jet spinning system for collecting fibers onto an array of rotating spindles for rapid tubular scaffold fabrication. The second team will focus on developing a perfusion bioreactor for long-term culture of stem cells in the tubular scaffolds. Strong synergy between these teams is needed for the overall success of the project.