Searching for Broad-Spectrum Antivirals Among Membrane-Active Molecules
University
              
          Sponsor
              Department of Energy
          Goals
              The goal of this project is to construct model membranes mimicking viral envelopes and characterize their interaction with antiviral peptides, amphiphiles and small molecules.
          Issues
          VIP students participating in this project will explore efficacies of membrane-targeting candidates for viral inhibition including viral peptides, amphiphiles, and membrane destabilizing small molecules. They will learn to prepare model membranes (e.g., giant vesicles), which mimic the viral envelope and mammalian cell membranes and using time-resolved fluorescence microscopies decipher in real-time how antiviral candidates destablize viral envelope, while leaving host cells unharmed. Students will acquire knowledge, understanding, and experiences of concepts, experimental methodologies, and characterization techniques from fields of Biophysics, Biomedical Engineering, Molecular and Cell biology, Chemistry and Biochemistry, and Materials Science & Engineering.
              Tools and Methods
          Giant unilamellar vesicle preparation
          Fluorescence microscopy
          Real time monitoring of vesicle-antiviral molecule interaction
          Modeling biomembrane organization and reorganization
              Desired Majors
          Biomedical engineering
          Chemical engineering
          Biochemistry
          Chemistry
          Immunology
          Physics
              Prep
              Students interested in molecular level understanding and characterization of biomembrane-antiviral molecule interactions. Basic knowledge in chemistry, biology, and physics principles and techniques will be helpful.
          Sponsor
              Department of Energy