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Searching for Broad-Spectrum Antivirals Among Membrane-Active Molecules

Sponsor
Department of Energy
Goals
The goal of this project is to construct model membranes mimicking viral envelopes and characterize their interaction with antiviral peptides, amphiphiles and small molecules.
Issues
VIP students participating in this project will explore efficacies of membrane-targeting candidates for viral inhibition including viral peptides, amphiphiles, and membrane destabilizing small molecules. They will learn to prepare model membranes (e.g., giant vesicles), which mimic the viral envelope and mammalian cell membranes and using time-resolved fluorescence microscopies decipher in real-time how antiviral candidates destablize viral envelope, while leaving host cells unharmed. Students will acquire knowledge, understanding, and experiences of concepts, experimental methodologies, and characterization techniques from fields of Biophysics, Biomedical Engineering, Molecular and Cell biology, Chemistry and Biochemistry, and Materials Science & Engineering.
Tools and Methods
Giant unilamellar vesicle preparation
Fluorescence microscopy
Real time monitoring of vesicle-antiviral molecule interaction
Modeling biomembrane organization and reorganization
Desired Majors
Biomedical engineering
Chemical engineering
Biochemistry
Chemistry
Immunology
Physics
Prep
Students interested in molecular level understanding and characterization of biomembrane-antiviral molecule interactions. Basic knowledge in chemistry, biology, and physics principles and techniques will be helpful.
Sponsor
Department of Energy