Structure-guided Vaccine Designs.

Goals

Design and structurally characerize enevelop proteins to target viruses such as HIV.

Issues Involved or Addressed

Recently the surface proteins of HIV, collectively known as envelop proteins, or Env, have generated promising results by their ability to increase the reactivity of the immune system cells, a process vital for eradicating the virus from the body. However, HIV-1 Env is highly dynamic and has high mutation rates, making vaccine design extremely challenging. A soluble form of Env, gp145 is a candidate for vaccine design. Therefore, structural characterization of gp145 is a crucial step in designing an immunogen-based vaccine. This protein will be expressed and characterized by electron microscopy and other studies.

Methods and Technologies

  • Cryo Electron Microscopy
  • Molecular modeling
  • Protein expression and purification
  • Optical microscopy

Academic Majors of Interest

  • Chemistry
  • Biochemistry
  • Microbiology and Immunology
  • Physics
  • Physiology
  • Computer Science

Preferred Interests and Preparation

An interest in elucidating the structure-function relationship of antiviral proteins. Basic understanding of molecular biology techniques, computer modeling, and microscopy.

Team Advisors

Holland Cheng

Sponsor(s)

NIH